Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
14.2K Views
•
10:05 min
•
May 23rd, 2020
DOI :
May 23rd, 2020
•Chapters in this video
0:04
Introduction
0:59
Postnatal Day 1-3 (P1-3) Mouse Brain Harvest
2:28
Subventricular Zone (SVZ)/Dentate Gyrus (DG) Microdissections
4:22
Tissue Dissociation
5:27
Postnatal Neural Stem Cell Expansion
6:09
Neurosphere Passaging
7:10
Results: Representative Neurosphere Expansion and Characterization
9:21
Conclusion
Related Videos
13:21
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
26.9K Views
09:24
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
29.7K Views
11:25
Isolation and Culture of Mouse Cortical Astrocytes
91.2K Views
10:51
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
13.1K Views
09:52
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
37.5K Views
09:24
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
15.0K Views
10:08
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
7.8K Views
00:06
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
5.8K Views
00:12
Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo
8.7K Views
07:04
Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum
5.8K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved