Zaloguj się
JoVE Journal
Biochemistry
This content is Open Access.
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
1.9K Views
•
08:33 min
•
March 11th, 2021
DOI :
March 11th, 2021
•Rozdziały w tym wideo
0:04
Introduction
0:36
Unroofed Membrane Experiments
4:36
Patch-Clamp Fluorometry Experiment
5:56
Results: Representative Measurement of Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
7:55
Conclusion
Powiązane Filmy
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
8.7K Views
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
13.1K Views
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
16.2K Views
Measuring Protein Binding to F-actin by Co-sedimentation
16.3K Views
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
8.2K Views
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
7.0K Views
Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
9.2K Views
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
14.2K Views
Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
6.8K Views
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
10.7K Views
ISSN 2578-2037
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone
Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.
Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.