RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Please note that all translations are AI generated. Click here for the English version.

7.5K Views

•

08:55 min

•

May 29th, 2020

DOI :

10.3791/61477-v

May 29th, 2020

•

Shubham Rathore¹, Jenni Hassert¹, Courtney M. Clark-Hachtel²^,³, Aaron Stahl¹^,⁴, Yoshinori Tomoyasu², Elke K. Bushbeck¹

¹Department of Biological Sciences, University of Cincinnati, ²Department of Biology, Miami University, ³Department of Biology, University of North Carolina at Chapel Hill, ⁴Department of Neuroscience, The Scripps Research Institute

Podsumowanie

Przeglądaj więcej filmów

Microinjection Technique

Stereo Microscope

Embryonic Manipulation

Injection Pressure

Rozdziały w tym wideo

0:04

Introduction

0:37

Early stage T. marmoratus Embryo Preparation

1:58

Early Stage T. marmoratus Embryo Double Stranded RNA (dsRNA) Microinjection

5:04

T. marmoratus Larva Preparation

5:58

T. marmoratus Larva dsRNA Microinjection

7:05

Results: Representative Effects of RNA Interference in Aquatic Beetles at Different Developmental Time Points

8:18

Conclusion

Powiązane Filmy

12:02

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

20.5K Views

08:16

Experimental Protocol for Manipulating Plant-induced Soil Heterogeneity

18.6K Views

08:09

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

8.7K Views

05:07

Alternative Method of Removing Otoliths from Sturgeon

8.3K Views

12:15

A Flow-through Exposure System for Evaluating Suspended Sediments Effects on Aquatic Life

8.3K Views

10:14

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

13.7K Views

08:30

Characterization of Aquatic Biofilms with Flow Cytometry

8.9K Views

07:47

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression

14.5K Views

08:33

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

11.0K Views

08:22

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

7.3K Views