Electroporation-mediated RNA Interference Method in Odonata

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5.6K Views

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13:28 min

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February 6th, 2021

DOI :

10.3791/61952-v

February 6th, 2021

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Genta Okude¹^,², Takema Fukatsu¹^,²^,³, Ryo Futahashi²

¹Department of Biological Sciences, Graduate School of Science, The University of Tokyo, ²Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), ³Graduate School of Life and Environmental Sciences, University of Tsukuba

Podsumowanie

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Ischnura Senegalensis

Electroporator

Larval Treatment

Inter segmental Membrane

Ultrasound Gel

Genetic Analysis

Rozdziały w tym wideo

0:00

Introduction

1:01

Protocol: Electroporation-mediated RNAi- In the Abdomen of Damselfly (Ischnura senegalensis)

4:21

Protocol: Electroporation-mediated RNAi- In the Thorax of Damselfly (Ischnura senegalensis)

6:54

Protocol: Electroporation-mediated RNAi- In the Abdomen of Dragonfly (Pseudothemis zonata)

10:16

Representative Results

11:51

Discussion

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