Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

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3.5K Views

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06:21 min

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October 6th, 2020

DOI :

10.3791/61662-v

October 6th, 2020

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David G. Keener*¹^,², Amy Cheung*¹^,³, Kensuke Futai¹

¹Brudnick Neuropsychiatric Research Institute, Department of Neurobiology, University of Massachusetts Medical School, ²Interdisciplinary Graduate Program, University of Massachusetts Medical School, ³UMMS MD/PhD Program, University of Massachusetts Medical School

Podsumowanie

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Single Cell Electroporation

Organotypic Slice Culture

Mouse Hippocampal Neurons

Gene Transfection

Excitatory Neurons

Inhibitory Neurons

Transfection Efficiency

Neurobiology Studies

Molecular Functions

Physiological Functions

Cell Autonomous Mechanisms

Transsynaptic Interactions

Electroporation Protocol

Petri Dishes

Culture Medium

Electroporator Settings

Plasmid Internal Solution

Rozdziały w tym wideo

0:04

Introduction

0:46

Electroporation Preparation

3:03

Electroporation

4:58

Results: Representative Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neuron Electroporation Efficiency

5:45

Conclusion

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