Gene transfection is a vital approach for neurobiology studies. Our electroporation technique enables us to transfect gene of interest interneurons in organotypic hippocampus slice culture with no detectable side effects. This protocol has a much
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Presented here is a protocol for single-cell electroporation that can deliver genes in both excitatory and inhibitory neurons across a range of in vitro hippocampal slice culture ages. Our approach provides precise and efficient expression of genes in individual cells, which can be used to examine cell-autonomous and intercellular functions.