Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
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7.4K Views
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09:59 min
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October 7th, 2020
DOI :
October 7th, 2020
•Juho-Antti Mäkelä1, Sheyla Cisneros-Montalvo1, Tiina Lehtiniemi1, Opeyemi Olotu1, Hue M. La3,4, Jorma Toppari1,2, Robin M. Hobbs3,4, Martti Parvinen1, Noora Kotaja1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia
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Rozdziały w tym wideo
0:04
Introduction
0:51
Testis Dissection and Preparation
2:10
Transillumination-Assisted Microdissection
2:47
Squash Preparation and Stage Verification
4:04
Whole-Mount Seminiferous Tubule Immunostaining
7:04
Results: Representative Mouse Seminiferous Epithelial Cycle Staging
9:16
Conclusion
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