Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

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6.0K Views

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08:32 min

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October 2nd, 2020

DOI :

10.3791/61805-v

October 2nd, 2020

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Margaret A. Nagai-Singer¹, Alissa Hendricks-Wenger¹^,²^,³, Rebecca M. Brock¹^,², Holly A. Morrison¹, Juselyn D. Tupik¹, Sheryl Coutermarsh-Ott¹, Irving C. Allen¹^,²^,⁴

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, ²Graduate Program in Translational Biology, Medicine and Health, Virginia Polytechnic Institute and State University, ³Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, ⁴Department of Basic Science Education, Virginia Tech Carilion School of Medicine, Virginia Polytechnic Institute and State University

Podsumowanie

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Computer based Image Analysis

Lung Metastasis

4T1 Breast Cancer Model

Quantification

Preclinical Research

Breast Cancer Therapies

Rozdziały w tym wideo

0:04

Introduction

0:51

Processing Tissues

2:49

Staining Plates

4:01

Image Analysis

6:26

Results: Fiji-ImageJ Analysis is Accurate and Precise in Determining Metastatic Burden

8:04

Conclusion

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