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Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

2.3K Views

09:20 min

July 6th, 2021

DOI :

10.3791/62109-v

July 6th, 2021


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Optogenetics

Rozdziały w tym wideo

0:04

Introduction

0:43

Gene Circuit Design

1:39

Stable Cell Line Engineering

2:27

Light Plate Apparatus Induction Assays

4:08

Fluorescence Microscopy of Light-Induced Engineered Cells

4:32

Flow Cytometry of Light-Induced Engineered Cells

5:14

Immunofluorescence of Gene Circuit Components

7:16

Results: Gene Expression Analysis in Engineered Optogenetic Cell Lines

8:49

Conclusion

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