Results: Identifying the Mutations Associated with Double Strand Break Repair near the CD4 Promoter
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Conclusion
Transcrição
This protocol helps you to identify mutations that occur during the repair of a double-strand break induced by single-guide RNA and CAS9. The main advantage of this technique is that it is a cost-effective and robust way to detect mutations at a s
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Presented here is sgRNA/CAS9 endonuclease and next-generation sequencing protocol that can be used to identify the mutations associated with double strand break repair near the CD4 promoter.