Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts

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1.6K Views

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07:16 min

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May 14th, 2021

DOI :

10.3791/62605-v

May 14th, 2021

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Lu Zhang¹, Wenwen Liu¹, Xifeng Wang¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Podsumowanie

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Immunofluorescent Labeling

Plant Virus

Insect Vector Proteins

Laser Scanning Confocal Microscopy

Gut Exposure Techniques

Rozdziały w tym wideo

0:04

Introduction

0:42

Virus Acquisition, Collection of Viruliferous Insects, and Reagent Preparation

1:24

Dissection of Adults and Excision of Guts

2:38

Dissection of Nymphs and Excision of Guts

3:47

Labeling Protocols for SRBSDV Virions and an Insect Protein

5:33

Results: Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts

6:32

Conclusion

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