Virus Acquisition, Collection of Viruliferous Insects, and Reagent Preparation
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Dissection of Adults and Excision of Guts
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Dissection of Nymphs and Excision of Guts
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Labeling Protocols for SRBSDV Virions and an Insect Protein
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Results: Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts
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Conclusion
副本
This protocol can be used to study various movement in insects, functions of insect proteins, and interactions between virus and vector insect in vivo. This method is efficient and informative. The structures of the insect gut and the auxiliary ce
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This protocol for immunofluorescent labeling of both plant virus proteins and vector insect proteins in excised insect guts can be used to study interactions among virus and vector insects, insect protein functions and molecular mechanisms underlying virus transmission.