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Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

2.3K Views

07:31 min

August 19th, 2021

DOI :

10.3791/62733-v

August 19th, 2021


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PH Measurement

Rozdziały w tym wideo

0:05

Introduction

0:28

Measuring Macropinosome pH

2:06

In Situ Calibration of Macropinosome pH

3:04

Measuring Oxidative Events within Macropinosomes

4:22

Measuring Protein Digestion within Macropinosomes

5:30

Results: Measuring the pH, Oxidative Events, and Protein Degradation in Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

6:59

Conclusion

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