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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

7.4K Views

12:15 min

February 21st, 2019

DOI :

10.3791/58896-v

February 21st, 2019


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Single Liposome Measurements

Rozdziały w tym wideo

0:04

Title

0:28

Preparation of a Lipid/UQ-10/FDLL Mix

1:26

Reconstitution of Cytochrome bo3

5:06

Fabrication of Semi-Transparent Gold Electrodes and Modification of the Gold Surface with Self-Assembled Monolayer (SAM)

6:07

Electrochemical Testing of Proteoliposomes Activity

7:46

Detection of Enzymatic Proton Pumping by Fluorescence Microscopy

9:44

Results: Liposome Identification and pH Change

11:35

Conclusion

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