Results: Measuring the pH, Oxidative Events, and Protein Degradation in Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
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Conclusion
Transcription
This protocol allows for the real-time assessment of various processes and biochemical parameters within the lumen of individual macropinosomes. These methods can be employed to drug discovery in the cell biology of macropinocytosis. This protocol
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We describe protocols for measuring pH, oxidative events, and protein digestion in individual macropinosomes in live cells. An emphasis is placed on dual-fluorophore ratiometric microscopy and the advantages it offers over population-based techniques.