Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
2.4K Views
•
11:44 min
•
January 19th, 2022
DOI :
January 19th, 2022
•Przeglądaj więcej filmów
Rozdziały w tym wideo
0:05
Introduction
0:52
Cell Homogenization
3:56
Cell Membrane Solubilization
4:46
Cell Membrane Immobilization on IAM.PC.DD2 Particles
6:23
CMAC Column Packing
8:57
Results: Antibody Staining and Frontal Affinity Chromatography of Immobilized TrkB Receptors
11:02
Conclusion
Powiązane Filmy
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
14.8K Views
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
13.1K Views
Assaying Protein Kinase Activity with Radiolabeled ATP
18.2K Views
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
34.7K Views
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
11.2K Views
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
8.5K Views
Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
17.1K Views
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
7.1K Views
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
10.1K Views
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
8.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone