Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

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1.8K Views

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09:37 min

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September 23rd, 2022

DOI :

10.3791/64671-v

September 23rd, 2022

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Linlin Zhang*¹^,², Meng Zhang*¹^,²^,³, Mengjia Liu¹^,², Junhong Ai¹^,², Jiao Tian¹^,², Huizheng Ge⁴, Ran Wang¹^,², Zhengde Xie¹^,²

¹Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, ²Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, ³Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, ⁴Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd

Podsumowanie

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Polyfunctional T Cells

Japanese Encephalitis Vaccine

Flow Cytometry

PBMCs

JEV Stimulation

Monoclonal Antibodies

Cell Surface Marker Staining

Fixation

Membrane breaking Fixative

Rozdziały w tym wideo

0:05

Introduction

0:39

Stimulation of PBMCs by Inactivated JEV Particles to Induce Cytokine Expression and Ex Vivo Intracellular Staining

4:28

Flow Cytometry Set-Up, Gating Strategy, and Data Analysis

6:56

Results: Flow Cytometry Analysis of Polyfunctional T Cell Profiles in PBMCs Within Japanese Encephalitis Virus Vaccinated Children

9:12

Conclusion

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