Stimulation of PBMCs by Inactivated JEV Particles to Induce Cytokine Expression and Ex Vivo Intracellular Staining
4:28
Flow Cytometry Set-Up, Gating Strategy, and Data Analysis
6:56
Results: Flow Cytometry Analysis of Polyfunctional T Cell Profiles in PBMCs Within Japanese Encephalitis Virus Vaccinated Children
9:12
Conclusion
Transkript
The detection method and the flow cytometry color scheme of Japanese encephalitis virus-specific TPFS were tested to provide a reference for similar studies. Demonstrating the procedure will be performed by Linlin Zhang and Meng Zhang, two student
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
The present protocol combines ex vivo stimulation and flow cytometry to analyze polyfunctional T cell (TPF) profiles in peripheral blood mononuclear cells (PBMCs) within Japanese encephalitis virus (JEV)-vaccinated children. The detection method and flow cytometry color scheme of JEV-specific TPFs were tested to provide a reference for similar studies.