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Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

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09:37 min

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September 23rd, 2022

DOI :

10.3791/64671-v

September 23rd, 2022

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1,735 Views

Linlin Zhang*¹^,², Meng Zhang*¹^,²^,³, Mengjia Liu¹^,², Junhong Ai¹^,², Jiao Tian¹^,², Huizheng Ge⁴, Ran Wang¹^,², Zhengde Xie¹^,²

¹Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, ²Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, ³Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, ⁴Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd

章

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要約

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Japanese Encephalitis Vaccine

Polyfunctional T Cells

Flow Cytometry

PBMC Stimulation

Cell Surface Marker Staining

Intracellular Cytokine Staining

Virus specific T Cells

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