Methods Article

Murine Dermal Lymphatic Endothelial Cell Isolation

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1.3K Views

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05:52 min

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July 21st, 2023

DOI :

10.3791/65393-v

July 21st, 2023

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Racheal Grace Akwii*¹, Margarita Lamprou*², Maria Georgomanoli³, Andriana Plevriti², Antonia Marazioti⁴, Athanasia Mouzaki⁵, George Mattheolabakis⁶, Constantinos M. Mikelis¹^,²

¹Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, ²Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, ³Division of Flow Cytometry and Division of MDx & Life Sciences, SB BioAnalytica, ⁴Basic Sciences Laboratory, Department of Physiotherapy, University of Peloponnese, ⁵Division of Hematology, Department of Internal Medicine, Medical School, University of Patras, ⁶School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana at Monroe

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Murine Dermal Lymphatic Endothelial Cells

Isolation Protocol

Magnetic Bead Purification

Lymphatic Endothelial Cells

Renal Endothelial Cells

Fluorescence activated Cell Sorting

Transgenic Mice

Lymphatic System

Immune Surveillance

Cre lox Technology

Gene Expression Levels

In Vitro Assays

Protein Identification

Standardized Method

Rozdziały w tym wideo

0:00

Introduction

0:58

Isolation of Cells from Murine Dermis

3:44

Murine Lymphatic Endothelial Cell Purification

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