This research is focused on establishing process for rapidly and efficiently isolating pure stage one ooyctes in zebrafish while eliminating granulosa cell contamination. The primary aim is to develop a method that facilities more precise analysis in oocyte specific research areas, particularly in genome and epigenetic research. The oocytes of zebrafish are enveloped by a mono-layer of granule cells.
It's difficult to swiftly separate granule cells from oocytes due to the significant disparity in both number and volume, making it challenging to obtain pure oocyte samples for specific analysis, especially in genome related studies. This method has survival advantage over mechanical methods and the previous detection approaches. First, the improved detection buffer is gentler, helping to isolate the granulosa cells while minimizing damage to the oocytes.
Plus, it allows us to get plenty of clean stage one oocytes. We hope as research helps scientists get a better look at the detail and growth of the phage oocytes, including cell stack, epigenetics and genome structure. This method work just as well for pompanos, and we think it could be applied to study a lot of different kind of fish.
