Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers
822 Views
•
09:15 min
•
July 19th, 2024
DOI :
July 19th, 2024
•Rozdziały w tym wideo
0:00
Introduction
2:08
Generating Single‐Cell Suspension from Lung Tissue for Identifying Rare Antigen‐Specific T‐Cell Populations
5:25
Enriching the Lung Sample for T Cells via Magnetic Bead Enrichment
7:40
Staining the Antigen‐Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting
Powiązane Filmy
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
19.5K Views
Retroviral Transduction of T-cell Receptors in Mouse T-cells
34.8K Views
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
11.6K Views
Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells
9.7K Views
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
10.9K Views
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
17.9K Views
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
8.4K Views
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
8.6K Views
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
11.3K Views
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
10.3K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone