Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

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09:15 min

July 19th, 2024

DOI :

10.3791/66939-v

July 19th, 2024


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Rare T Cells

Rozdziały w tym wideo

0:00

Introduction

2:08

Generating Single‐Cell Suspension from Lung Tissue for Identifying Rare Antigen‐Specific T‐Cell Populations

5:25

Enriching the Lung Sample for T Cells via Magnetic Bead Enrichment

7:40

Staining the Antigen‐Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting

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