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Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

8.2K Views

08:10 min

November 28th, 2018

DOI :

10.3791/58680-v

November 28th, 2018


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Simultaneous Quantification

Rozdziały w tym wideo

0:04

Title

0:49

Sample Collection

1:21

Staining Set-up

1:59

Tetramer Staining and Preparation of Antibodies

3:17

Staining of Samples

4:24

Lysis of Erythrocytes

5:09

Flow Cytometric Measurement and Analysis

6:53

Results: Quantification of Antigen-specific CD8+ T Cells in Mouse Blood of Lymphoid Organs

7:36

Conclusion

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