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Development and Application of Rapamycin-regulated Tyrosine Phosphatases

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06:56 min

September 6th, 2024

DOI :

10.3791/67142-v

September 6th, 2024


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Rapamycin

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0:00

Introduction

1:39

Preparing Cells Expressing Rapamycin‐Regulated Phosphatase for Immunoprecipitation to Assess Temporal Control of Tyrosine Phosphatases

3:36

Immunoprecipitation of RapR‐Shp2 to Study Chemogenetic Control of Tyrosine Phosphatase

4:52

Phosphatase Activity Assay to Characterize Allosteric Regulation of Phosphatases with the Rapamycin‐Regulated RapR System

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