Stuart H. Orkin

Functional overlap of GATA-1 and GATA-2 in primitive hematopoietic development.

The zinc-finger proto-oncogene Gfi-1b is essential for development of the erythroid and megakaryocytic lineages.

The search for the hemangioblast.

Hematopoiesis and stem cells: plasticity versus developmental heterogeneity.

Interaction between FOG-1 and the corepressor C-terminal binding protein is dispensable for normal erythropoiesis in vivo.

Distinct domains of the GATA-1 cofactor FOG-1 differentially influence erythroid versus megakaryocytic maturation.

Transcriptional regulation of erythropoiesis: an affair involving multiple partners.

Targeted deletion of a high-affinity GATA-binding site in the GATA-1 promoter leads to selective loss of the eosinophil lineage in vivo.

The use of altered specificity mutants to probe specific protein-protein interactions involved in the activation of GATA-1 target genes.

GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis.

Stem-cell competition.

X-linked thrombocytopenia with thalassemia from a mutation in the amino finger of GATA-1 affecting DNA binding rather than FOG-1 interaction.

Dysregulation of protein modification by ISG15 results in brain cell injury.

Gonadal differentiation, sex determination and normal Sry expression in mice require direct interaction between transcription partners GATA4 and FOG2.

The SCL complex regulates c-kit expression in hematopoietic cells through functional interaction with Sp1.

Expression of CD41 marks the initiation of definitive hematopoiesis in the mouse embryo.

The zinc finger transcription factor Gfi1, implicated in lymphomagenesis, is required for inner ear hair cell differentiation and survival.

Highly penetrant, rapid tumorigenesis through conditional inversion of the tumor suppressor gene Snf5.

Intrinsic requirement for zinc finger transcription factor Gfi-1 in neutrophil differentiation.

Haematopoietic stem cells retain long-term repopulating activity and multipotency in the absence of stem-cell leukaemia SCL/tal-1 gene.

GATA-1 as a regulator of mast cell differentiation revealed by the phenotype of the GATA-1low mouse mutant.

Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression.

Combinatorial interactions of serpent, lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis.

Endothelial lineage-mediated loss of the GATA cofactor Friend of GATA 1 impairs cardiac development.

Priming the hematopoietic pump.

Coregulator-dependent facilitation of chromatin occupancy by GATA-1.

More than blood, a novel gene required for mammalian postimplantation development.

The SWI/SNF complex--chromatin and cancer.

Defective neural tube closure and anteroposterior patterning in mice lacking the LIM protein LMO4 or its interacting partner Deaf-1.

Transforming acidic coiled-coil protein 3 (TACC3) controls friend of GATA-1 (FOG-1) subcellular localization and regulates the association between GATA-1 and FOG-1 during hematopoiesis.

Endogenous oncogenic K-ras(G12D) stimulates proliferation and widespread neoplastic and developmental defects.

Decoding hematopoietic specificity in the helix-loop-helix domain of the transcription factor SCL/Tal-1.

The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis.

Tel/Etv6 is an essential and selective regulator of adult hematopoietic stem cell survival.

A critical role for eosinophils in allergic airways remodeling.

Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells.

GATA-2 plays two functionally distinct roles during the ontogeny of hematopoietic stem cells.

Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library.

Gene targeting and transgenic strategies for the analysis of hematopoietic development in the mouse.

Enforced expression of PU.1 rescues osteoclastogenesis from embryonic stem cells lacking Tal-1.

Coregulation of GATA factors by the Friend of GATA (FOG) family of multitype zinc finger proteins.

Tie2Cre-mediated gene ablation defines the stem-cell leukemia gene (SCL/tal1)-dependent window during hematopoietic stem-cell development.

The placenta is a niche for hematopoietic stem cells.

Haploinsufficiency of GATA-2 perturbs adult hematopoietic stem-cell homeostasis.

Early asymmetry of gene transcription in embryonic human left and right cerebral cortex.

Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1.

Stem cells: the road not taken.

The transcription factor GATA2 regulates differentiation of brown adipocytes.

Phosphatidylinositol 3-kinase/Akt induced by erythropoietin renders the erythroid differentiation factor GATA-1 competent for TIMP-1 gene transactivation.

Placenta as a site for hematopoietic stem cell development.

Chipping away at the embryonic stem cell network.

Specification of astrocytes by bHLH protein SCL in a restricted region of the neural tube.

Zinc-finger transcription factor Gfi-1: versatile regulator of lymphocytes, neutrophils and hematopoietic stem cells.

Phosphorylation of Gata1 at serine residues 72, 142, and 310 is not essential for hematopoiesis in vivo.

Reactions to the Hwang scandal.

Identification of distinct molecular phenotypes in acute megakaryoblastic leukemia by gene expression profiling.

Characterization of a megakaryocyte-specific enhancer of the key hemopoietic transcription factor GATA1.

Notch signaling requires GATA-2 to inhibit myelopoiesis from embryonic stem cells and primary hemopoietic progenitors.

Rb is dispensable for self-renewal and multilineage differentiation of adult hematopoietic stem cells.

Unraveling the transcriptional network controlling ES cell pluripotency.

The journey of developing hematopoietic stem cells.

The hypomorphic Gata1low mutation alters the proliferation/differentiation potential of the common megakaryocytic-erythroid progenitor.

A protein interaction network for pluripotency of embryonic stem cells.

Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart.

Gata4 is necessary for normal pulmonary lobar development.

Rb regulates interactions between hematopoietic stem cells and their bone marrow microenvironment.

Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis.

Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1.

Immunology: changed destiny.

ETV6-NTRK3 fusion oncogene initiates breast cancer from committed mammary progenitors via activation of AP1 complex.

Site-directed, virus-free, and inducible RNAi in embryonic stem cells.

A protein roadmap to pluripotency and faithful reprogramming.

Genome-wide association study shows BCL11A associated with persistent fetal hemoglobin and amelioration of the phenotype of beta-thalassemia.

Rb intrinsically promotes erythropoiesis by coupling cell cycle exit with mitochondrial biogenesis.

Oct4 dependence of chromatin structure within the extended Nanog locus in ES cells.

Hematopoiesis: an evolving paradigm for stem cell biology.

SnapShot: hematopoiesis.

Antagonism of FOG-1 and GATA factors in fate choice for the mast cell lineage.

PRC1 and Suv39h specify parental asymmetry at constitutive heterochromatin in early mouse embryos.

An extended transcriptional network for pluripotency of embryonic stem cells.

The emergence of hematopoietic stem cells is initiated in the placental vasculature in the absence of circulation.

Targeted disruption of the basic Krüppel-like factor gene (Klf3) reveals a role in adipogenesis.

Stem cells down under-ISSCR 2007.

Requirement of Nanog dimerization for stem cell self-renewal and pluripotency.

Conditional mouse osteosarcoma, dependent on p53 loss and potentiated by loss of Rb, mimics the human disease.

DNA polymorphisms at the BCL11A, HBS1L-MYB, and beta-globin loci associate with fetal hemoglobin levels and pain crises in sickle cell disease.

GATA4 mediates gene repression in the mature mouse small intestine through interactions with friend of GATA (FOG) cofactors.

Rb and hematopoiesis: stem cells to anemia.

Polycomb group proteins Ezh2 and Rnf2 direct genomic contraction and imprinted repression in early mouse embryos.

EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency.

Human fetal hemoglobin expression is regulated by the developmental stage-specific repressor BCL11A.

ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling.

Glimpses of the epigenetic landscape.

Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis.

Tandem affinity purification of protein complexes in mouse embryonic stem cells using in vivo biotinylation.

Use of in vivo biotinylation to study protein-protein and protein-DNA interactions in mouse embryonic stem cells.

A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.

Musings on genome medicine: abuse of genetic tests.

Musings on genome medicine: the Obama effect.

Musings on genome medicine: genome wide association studies.

Musings on genome medicine: gene therapy.

Musings on genome medicine: cancer genetics and the promise of effective treatment.

Musings on genome medicine: cholesterol and coronary artery disease.

TEL-AML1 corrupts hematopoietic stem cells to persist in the bone marrow and initiate leukemia.

GATA-2 reinforces megakaryocyte development in the absence of GATA-1.

Translational isoforms of FOG1 regulate GATA1-interacting complexes.

Developmental and species-divergent globin switching are driven by BCL11A.

Musings on genome medicine: the Obama effect redux.

DNA methyltransferase 1 is essential for and uniquely regulates hematopoietic stem and progenitor cells.

Musings on genome medicine: the slow but inexorable process of medical care reform in the United States.

Musings on genome medicine: Crohn's disease.

Insights into GATA-1-mediated gene activation versus repression via genome-wide chromatin occupancy analysis.

Musings on genome medicine: enzyme-replacement therapy of the lysosomal storage diseases.

Jumonji modulates polycomb activity and self-renewal versus differentiation of stem cells.

Systematic tracking of cell fate changes.

Perturbed hematopoiesis in the Tc1 mouse model of Down syndrome.

Musings on genome medicine: Hepatitis C.

miR-125b-2 is a potential oncomiR on human chromosome 21 in megakaryoblastic leukemia.

Advances in the understanding of haemoglobin switching.

DNA methylation in adult stem cells: new insights into self-renewal.

Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6.

Sumoylation regulates interaction of FOG1 with C-terminal-binding protein (CTBP).

TIF1gamma controls erythroid cell fate by regulating transcription elongation.

Medicine. Sickle cell disease at 100 years.

Developmental stage-specific interplay of GATA1 and IGF signaling in fetal megakaryopoiesis and leukemogenesis.

Transcriptional silencing of fetal hemoglobin by BCL11A.

Training program in cancer and blood diseases: Pediatric Hematology/Oncology Fellowship Program, Children's Hospital Boston/Dana-Farber Cancer Institute.

Differential roles of Sall4 isoforms in embryonic stem cell pluripotency.

A single cis element maintains repression of the key developmental regulator Gata2.

A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs.

Epigenetic antagonism between polycomb and SWI/SNF complexes during oncogenic transformation.

Modifier genes in Mendelian disorders: the example of hemoglobin disorders.

Fine-mapping at three loci known to affect fetal hemoglobin levels explains additional genetic variation.

Networking erythropoiesis.

Therapeutic levels of fetal hemoglobin in erythroid progeny of β-thalassemic CD34+ cells after lentiviral vector-mediated gene transfer.

Update on fetal hemoglobin gene regulation in hemoglobinopathies.

MicroRNA-15a and -16-1 act via MYB to elevate fetal hemoglobin expression in human trisomy 13.

Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells.

Context-dependent function of "GATA switch" sites in vivo.

The erythroid/myeloid lineage fate paradigm takes a new player.

Genome Medicine: stem cells, genomics and translational research.

Chromatin connections to pluripotency and cellular reprogramming.

Loss of the retinoblastoma binding protein 2 (RBP2) histone demethylase suppresses tumorigenesis in mice lacking Rb1 or Men1.

MicroRNA-21 limits in vivo immune response-mediated activation of the IL-12/IFN-gamma pathway, Th1 polarization, and the severity of delayed-type hypersensitivity.

A functional element necessary for fetal hemoglobin silencing.

Zfp281 functions as a transcriptional repressor for pluripotency of mouse embryonic stem cells.

Correction of sickle cell disease in adult mice by interference with fetal hemoglobin silencing.

Embryonic stem cell-specific signatures in cancer: insights into genomic regulatory networks and implications for medicine.

Polycomb repressive complex 2 regulates normal development of the mouse heart.

Dnmt3a silences hematopoietic stem cell self-renewal.

PRC2 directly methylates GATA4 and represses its transcriptional activity.

A human stem cell model of early Alzheimer's disease pathology in Down syndrome.

MicroRNA-21 promotes fibrosis of the kidney by silencing metabolic pathways.

Haploinsufficiency of Dnmt1 impairs leukemia stem cell function through derepression of bivalent chromatin domains.

MicroRNA-21 integrates pathogenic signaling to control pulmonary hypertension: results of a network bioinformatics approach.

Polycomb repressive complex 2 is required for MLL-AF9 leukemia.

MAnorm: a robust model for quantitative comparison of ChIP-Seq data sets.

Exome sequencing identifies GATA1 mutations resulting in Diamond-Blackfan anemia.

The CACCC-binding protein KLF3/BKLF represses a subset of KLF1/EKLF target genes and is required for proper erythroid maturation in vivo.

Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium.

Reawakening fetal hemoglobin: prospects for new therapies for the β-globin disorders.

Cyclin D3 coordinates the cell cycle during differentiation to regulate erythrocyte size and number.

Combinatorial assembly of developmental stage-specific enhancers controls gene expression programs during human erythropoiesis.

Altered hematopoiesis in trisomy 21 as revealed through in vitro differentiation of isogenic human pluripotent cells.

TiF1-gamma plays an essential role in murine hematopoiesis and regulates transcriptional elongation of erythroid genes.

The switch from fetal to adult hemoglobin.

Sustained PU.1 levels balance cell-cycle regulators to prevent exhaustion of adult hematopoietic stem cells.

Genome-wide association studies of hematologic phenotypes: a window into human hematopoiesis.

ETV1 directs androgen metabolism and confers aggressive prostate cancer in targeted mice and patients.

Corepressor-dependent silencing of fetal hemoglobin expression by BCL11A.

Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation.

Delineating the mixed lineage leukemia gene expression network in hematopoietic stem cells.

Targeted disruption of the EZH2-EED complex inhibits EZH2-dependent cancer.

Mapping cellular hierarchy by single-cell analysis of the cell surface repertoire.

An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level.

Polycomb repressive complex 2 regulates normal hematopoietic stem cell function in a developmental-stage-specific manner.

Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity.

Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation.

Analysis of chromatin-state plasticity identifies cell-type-specific regulators of H3K27me3 patterns.

Corepressor Rcor1 is essential for murine erythropoiesis.

Distinct, strict requirements for Gfi-1b in adult bone marrow red cell and platelet generation.

Reprogramming committed murine blood cells to induced hematopoietic stem cells with defined factors.

Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells.

TMEM14C is required for erythroid mitochondrial heme metabolism.

Myeloproliferative neoplasms can be initiated from a single hematopoietic stem cell expressing JAK2-V617F.

Inflammatory signaling regulates embryonic hematopoietic stem and progenitor cell production.

A comparative encyclopedia of DNA elements in the mouse genome.

Mouse regulatory DNA landscapes reveal global principles of cis-regulatory evolution.

Complementary genomic approaches highlight the PI3K/mTOR pathway as a common vulnerability in osteosarcoma.

Characterizing heterogeneity in leukemic cells using single-cell gene expression analysis.