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NCR Biotech Science Cluster

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage
Jayesh Kumar Sevak 1, Suchitra D. Gopinath 1
1Translational Health Science and Technology Institute (THSTI), NCR Biotech Science Cluster

We describe a protocol for the isolation of mesenchymal stem cells from human umbilical cord tissue and their differentiation into the skeletal muscle lineage.

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Immunology and Infection

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Praapti Jayaswal 1, Mohd Ilyas 1, Kuljit Singh 1,2, Saurabh Kumar 1,3, Lovely Sisodiya 1, Sapna Jain 1, Rahul Mahlawat 1, Nishant Sharma 1,4, Vishal Gupta 1, Krishnamohan Atmakuri 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.

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