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NCR Biotech Science Cluster

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage

Jayesh Kumar Sevak¹, Suchitra D. Gopinath¹

¹Translational Health Science and Technology Institute (THSTI), NCR Biotech Science Cluster

We describe a protocol for the isolation of mesenchymal stem cells from human umbilical cord tissue and their differentiation into the skeletal muscle lineage.

Immunology and Infection

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Praapti Jayaswal¹, Mohd Ilyas¹, Kuljit Singh^1,2, Saurabh Kumar^1,3, Lovely Sisodiya¹, Sapna Jain¹, Rahul Mahlawat¹, Nishant Sharma^1,4, Vishal Gupta¹, Krishnamohan Atmakuri¹

¹Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, ²Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, ³ICAR-Research Complex for Eastern Region, ⁴Public Health Research Institute, Rutgers University

This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.