Ochsner Medical Center
2 ARTICLES PUBLISHED IN JoVE
Medicine
Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm
Zachary Tyerman 1, Jolian Dahl 1, Alexander Shannon 1, W. Forrest Johnston 2, Nicholas H. Pope 1, Guanyi Lu 3, Gilbert R. Upchurch Jr. 3, Gorav Ailawadi 1,4, Morgan Salmon 1
1Department of Surgery, University of Virginia School of Medicine, 2Department of Surgery, Ochsner Medical Center, 3Department of Surgery, University of Florida, 4The Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine
We describe a surgical protocol to consistently induce robust descending thoracic aortic aneurysms in mice. The procedure involves left thoracotomy, thoracic aorta exposure, and placement of a sponge soaked in porcine pancreatic elastase on the aortic wall.
Medicine
Porcine Model of Infrarenal Abdominal Aortic Aneurysm
Alexander H. Shannon 1, J. Michael Cullen 1, Jolian J. Dahl 1, Erik J. Scott 1, Zachary Tyerman 1, Michael D. Spinosa 1, William G. Montgomery 1, W. Forrest Johnston 2, Guanyi Lu 3, Morgan Salmon *1, Gorav Ailawadi *1,4, Gilbert R. Upchurch Jr. *3
1Department of Surgery, University of Virginia School of Medicine, 2Department of Surgery, Ochsner Medical Center, 3Department of Surgery, University of Florida, 4The Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine
This novel model creates robust infrarenal abdominal aortic aneurysms in swine using a combination of balloon angioplasty, elastase/collagenase perfusion, topical elastase application, and oral compound β-aminopropionitrile administration, which interferes with collagen cross-linking.
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