Accedi

Ochsner Medical Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

Zachary Tyerman¹, Jolian Dahl¹, Alexander Shannon¹, W. Forrest Johnston², Nicholas H. Pope¹, Guanyi Lu³, Gilbert R. Upchurch Jr.³, Gorav Ailawadi^1,4, Morgan Salmon¹

¹Department of Surgery, University of Virginia School of Medicine, ²Department of Surgery, Ochsner Medical Center, ³Department of Surgery, University of Florida, ⁴The Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

We describe a surgical protocol to consistently induce robust descending thoracic aortic aneurysms in mice. The procedure involves left thoracotomy, thoracic aorta exposure, and placement of a sponge soaked in porcine pancreatic elastase on the aortic wall.

Medicine

Porcine Model of Infrarenal Abdominal Aortic Aneurysm

Alexander H. Shannon¹, J. Michael Cullen¹, Jolian J. Dahl¹, Erik J. Scott¹, Zachary Tyerman¹, Michael D. Spinosa¹, William G. Montgomery¹, W. Forrest Johnston², Guanyi Lu³, Morgan Salmon^*1, Gorav Ailawadi^*1,4, Gilbert R. Upchurch Jr.^*3

This novel model creates robust infrarenal abdominal aortic aneurysms in swine using a combination of balloon angioplasty, elastase/collagenase perfusion, topical elastase application, and oral compound β-aminopropionitrile administration, which interferes with collagen cross-linking.