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University of Ghent

4 ARTICLES PUBLISHED IN JoVE

Medicine

The In ovo CAM-assay as a Xenograft Model for Sarcoma

Gwen M.L. Sys¹, Lore Lapeire², Nikita Stevens¹, Herman Favoreel³, Ramses Forsyth⁴, Marc Bracke², Olivier De Wever²

¹Department of Orthopaedic Surgery and Traumatology, Ghent University Hospital, ²Department of Radiation Oncology and Experimental Cancer Research, Ghent University, ³Department of Virology, Parasitology, and Immunology, Ghent University, ⁴Pathlicon

The in ovo chorioallantoic membrane (CAM) is grafted with fresh sarcoma-derived tumor tissues, their single cell suspensions, and permanent and transient fluorescently labeled established sarcoma cell lines. The model is used to study graft- (viability, Ki67 proliferation index, necrosis, infiltration) and host (fibroblast infiltration, vascular ingrowth) behavior.

Medicine

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Marc E. Bracke¹, Bart I. Roman², Christian V. Stevens², Liselot M. Mus¹, Virinder S. Parmar³, Olivier De Wever¹, Marc M. Mareel¹

¹Department of Radiation Oncology and Experimental Cancer Research, University of Ghent, ²Department of Sustainable Organic Chemistry and Technology, University of Ghent, ³Department of Chemistry, University of Delhi

Here, we present a protocol to study the invasion of tumor cells into living normal tissue fragments in three dimensions. This organ culture technique is mainly applied to test potentially anti-invasive drugs in vitro.

Bioengineering

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Britt Van Aelst¹, Hendrik B. Feys¹, Rosalie Devloo¹, Philippe Vandekerckhove^2,3,4, Veerle Compernolle^1,2,4

¹Transfusion Research Center, Belgium Red Cross-Flanders, ²Blood Service, Belgium Red Cross-Flanders, ³Department of Public Health and Primary Care, Catholic University of Leuven, ⁴Faculty of Medicine and Health Sciences, University of Ghent

Platelet transfusion and hemostasis was modeled using blood reconstitution and microfluidic flow chambers to investigate the function of blood banking platelets. The data demonstrate the consequences of platelet storage lesion on hemostasis, in vitro.

Bioengineering

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Katrijn R. Six^1,2, Rosalie Devloo¹, Britt Van Aelst¹, Philippe Vandekerckhove^2,3,4, Hendrik B. Feys¹, Veerle Compernolle^1,2,3

¹Transfusion Research Center, Belgian Red Cross-Flanders, ²Faculty of Medicine and Health Sciences, Ghent University, ³Blood Service, Belgian Red Cross-Flanders, ⁴Department of Public Health and Primary Care, KULeuven - University of Leuven

This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.