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Second Military Medical University

4 ARTICLES PUBLISHED IN JoVE

Medicine

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

Kezhe Tan^*1,2, Zhaoping Ding^*1,2, Bodo Steckel², Sonja Hartwig³, Stefan Lehr³, Xiaoming Deng¹, Jürgen Schrader²

¹Department of Anesthesiology, Changhai Hospital, Second Military Medical University, ²Department of Molecular Cardiology, Heinrich Heine University, ³Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Heinrich Heine University

This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.

Developmental Biology

Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland

Dongmei Cao¹, Xianhua Ma¹, Weiping J. Zhang¹, Zhifang Xie^1,2

¹Department of Pathophysiology, Second Military Medical University, ²Department of Cell Biology, Second Military Medical University

We present a protocol to dissect pituitary glands and prepare pituitary coronal sections from developing mice.

Behavior

Assessing the Autonomic and Behavioral Effects of Passive Motion in Rats using Elevator Vertical Motion and Ferris-Wheel Rotation

Francis A. M. Manno^1,2, Leilei Pan³, Yuqi Mao³, Yang Su³, Sinai H. C. Manno^2,4,5, Shuk Han Cheng^4,5,6, Condon Lau², Yiling Cai³

¹School of Biomedical Engineering, Faculty of Engineering, University of Sydney, ²Department of Physics, City University of Hong Kong, ³Department of Nautical Injury Prevention, Faculty of Navy Medicine, Second Military Medical University, ⁴State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, ⁵Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, ⁶Department of Materials Science and Engineering, City University of Hong Kong

Protocols are presented to assess the autonomic and behavioral effects of passive motion in rodents using elevator vertical motion and Ferris-wheel rotation.

Immunology and Infection

Clarifying and Imaging Candida albicans Biofilms

Frederick Lanni¹, Katherine Lagree¹, Manning Y. Huang¹, Lan Yan², Carol A. Woolford¹, Aaron P. Mitchell¹

¹Department of Biological Sciences, Carnegie Mellon University, ²Department of Pharmacology, Second Military Medical University

To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.