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Ruhr-University Bochum

10 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
Rebecca Conrad 1, Sibylle Jablonka 2, Teresa Sczepan 1, Michael Sendtner 2, Stefan Wiese 1, Alice Klausmeyer 1
1Institute for Cellmorphology and molecular Neurobiology, Group for Cellbiology, Ruhr-University Bochum, 2Institute for Clinical Neurobiology, University of Wuerzburg

An alternative way of isolating mouse embryonic motoneurons from the spinal cord is described. The method takes into account the fact that lectin can bind to the low affinity nerve growth factor receptor p75NTR. This lectin-based preplating allows a purification similar to that with a specific antibody against the p75NTR.

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Neuroscience

Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning
Sarah Starosta *1, Maik C. Stüttgen *1, Onur Güntürkün 1
1Faculty of Psychology, Department of Biopsychology, Ruhr-University Bochum

Learning new stimulus-response associations engages a wide range of neural processes which are ultimately reflected in changing spike output of individual neurons. Here we describe a behavioral protocol allowing for the continuous registration of single-neuron activity while animals acquire, extinguish, and reacquire a conditioned response within a single experimental session.

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Medicine

In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging
Angelika Hoffmann 1,2, Xavier Helluy 2,3, Manuel Fischer 2, Ann-Kristin Mueller 4,5, Sabine Heiland 2, Mirko Pham 1,6, Martin Bendszus 1, Johannes Pfeil 4,5,7
1Department of Neuroradiology, Heidelberg University Hospital, 2Division of Experimental Radiology, Department of Neuroradiology, Heidelberg University Hospital, 3NeuroImaging Centre Research, Department of Neuroscience, Ruhr-University Bochum, 4Centre for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, 5German Centre for Infection Research (DZIF), 6Department of Neuroradiology, University of Würzburg, 7Center for Childhood and Adolescent Medicine, General Pediatrics, Heidelberg University Hospital

We describe a mouse model of experimental cerebral malaria and show how inflammatory and microvascular pathology can be tracked in vivo using magnetic resonance imaging.

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Chemistry

Simple Methods for the Preparation of Non-noble Metal Bulk-electrodes for Electrocatalytic Applications
Kai junge Puring 1,2, Stefan Piontek 1, Mathias Smialkowski 1, Jens Burfeind 2, Stefan Kaluza 2, Christian Doetsch 2, Ulf-Peter Apfel 1
1Faculty of Chemistry and Biochemistry, Inorganic Chemistry I, Ruhr-University Bochum, 2Fraunhofer Institute for Environmental, Safety, and Energy Technology, UMSICHT

A facile preparation method of electrodes using the bulk material Fe4.5Ni4.5S8 is presented. This method provides an alternative technique to conventional electrode fabrication and describes prerequisites for unconventional electrode materials including a straightforward electrocatalytic testing method.

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Biology

Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
Felix M. Fuchs 1, Marina Raguse 1,2,3, Marcel Fiebrandt 2, Kazimierz Madela 4, Peter Awakowicz 2, Michael Laue 4, Katharina Stapelmann 3, Ralf Moeller 1
1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

This protocol illustrates the important consecutive steps required to assess the relevance of monitoring vitality parameter and DNA repair processes in reviving Bacillus subtilis spores after treatment with low pressure plasma by tracking fluorescence-labelled DNA repair proteins via time-resolved confocal microscopy and scanning electron microscopy.

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Neuroscience

Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats
Michael Dietrich 1, Christina Hecker 1, Alexander Hilla 2, Andrés Cruz-Herranz 3, Hans-Peter Hartung 1, Dietmar Fischer 2, Ari Green 3, Philipp Albrecht 1
1Department of Neurology, Heinrich-Heine-University Düsseldorf, 2Department of Cell Physiology, Faculty of Biology and Biotechnology, Ruhr-University Bochum, 3Division of Neuroinflammation and Glial Biology, Department of Neurology, University of California San Francisco

A detailed protocol for the assessment of structural and visual readouts in rodents by optical coherence tomography and optokinetic response is presented. The results provide valuable insights for ophthalmologic as well as neurologic research.

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Engineering

Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet
Judith Golda 1, Kerstin Sgonina 1, Julian Held 2, Jan Benedikt 1, Volker Schulz-von der Gathen 2
1Experimental Plasma Physics, Kiel University, 2Experimental Physics II, Ruhr-University Bochum

This protocol is presented to characterize the setup, handling, and application of the COST-Jet for the treatment of diverse surfaces such as solids and liquids.

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Medicine

Nerve Ultrasound Protocol to Detect Dysimmune Neuropathies
Anna Lena Fisse 1,2, Kalliopi Pitarokoili 1,2, Ralf Gold 1,2
1Department of Neurology, St. Josef-Hospital, Ruhr-University Bochum, 2Immunmediated Neuropathies Biobank (INHIBIT), Ruhr-University Bochum

This article presents a protocol for nerve ultrasound in polyneuropathies to aid the diagnosis of inflammatory neuropathies.

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Neuroscience

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
Maximilian Wulf 1,2, Katalin Barkovits-Boeddinghaus 1,2, Paula Sommer 1,2, Karin Schork 1,2, Martin Eisenacher 1,2, Peter Riederer 3,4, Manfred Gerlach 5, Steffen Kösters 1,2, Britta Eggers *1,2, Katrin Marcus *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg

A robust protocol is presented here for isolating neuromelanin granules from human post-mortem substantia nigra pars compacta tissue via laser microdissection. This revised and optimized protocol massively minimizes the required time for sample collection, reduces the required sample amount, and enhances the identification and quantification of proteins by LC-MS/MS analysis.

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Medicine

Performing Repeated Intraoperative Impedance Telemetry Measurements during Cochlear Implantation
Nora M. Weiss 1,2,3,4,5, Stefan Hans 6, Martin Wozniak 6, Aline Föger 6, Stefan Dazert 1, Vincent Van Rompaey 2,7, Paul Van de Heyning 2,7, Joachim Schmutzhard 8, Angelika Dierker 6
1Department of Otorhinolaryngology-Head and Neck Surgery, Ruhr-University Bochum, St. Elisabeth-Hospital Bochum, 2Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, 3International Graduate School of Neuroscience (IGSN), Ruhr-University Bochum, 4Technical University of Munich, 5Department of Otorhinolaryngology, TUM School of Medicine, Klinikum rechts der Isar, 6MED-EL, 7Department of Otorhinolaryngology and Head & Neck Surgery, Antwerp University Hospital, 8Department of Otorhinolaryngology-Head and Neck Surgery, Medical University Innsbruck

Here we present a protocol to conduct repeated impedance telemetry measurements during cochlear implantation (CI). They may allow conclusions on the electrode's and implant's function. Repeated impedance measurements enable objective feedback on whether the electrode is positioned inside the perilymph or outside the inner ear.

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