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Tokyo Metropolitan Institute of Medical Science

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

Pentylenetetrazole-Induced Kindling Mouse Model

¹Synaptic Plasticity Project, Tokyo Metropolitan Institute of Medical Science

This protocol describes a method of chemical kindling with pentylenetetrazole and provides a mouse model of epilepsy. This protocol can also be used to investigate vulnerability to seizure induction and pathogenesis after epileptic seizures in mice.

Neuroscience

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Taisuke Yoneda¹, Seiichiro Sakai^1,2, Hisato Maruoka¹, Toshihiko Hosoya¹

¹RIKEN Brain Science Institute, ²Tokyo Metropolitan Institute of Medical Science

Here we describe a procedure for tissue clearing, fluorescent labeling, and large-scale imaging of mouse brain tissue which, thereby, enables visualization of the three-dimensional organization of cell types in the neocortex.