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San Francisco State University

5 ARTICLES PUBLISHED IN JoVE

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Medicine

Myo-mechanical Analysis of Isolated Skeletal Muscle
Peter E. Oishi 1,2, Sompob Cholsiripunlert 3, Wenhui Gong 2, Anthony J. Baker 4, Harold S. Bernstein 1,2,5
1Cardiovascular Research Institute, University of California San Francisco, 2Department of Pediatrics, University of California San Francisco, 3Department of Biology, San Francisco State University, 4Department of Medicine, University of California San Francisco , 5Eli and Edythe Broad Center of Regeneration Medicine & Stem Cell Research, University of California San Francisco

To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.

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Engineering

Using Microwave and Macroscopic Samples of Dielectric Solids to Study the Photonic Properties of Disordered Photonic Bandgap Materials
Seyed Reza Hashemizad 1, Sam Tsitrin 1, Polin Yadak 1, Yingquan He 1, Daniel Cuneo 1, Eric Paul Williamson 1, Devin Liner 1, Weining Man 1
1Department of Physics and Astronomy, San Francisco State University

Disordered structures offer new mechanisms for forming photonic bandgaps and unprecedented freedom in functional-defect designs. To circumvent the computational challenges of disordered systems, we construct modular macroscopic samples of the new class of PBG materials and use microwaves to characterize their scale-invariant photonic properties, in an easy and inexpensive manner.

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Developmental Biology

Methods for the Study of Regeneration in Stentor
Athena Lin 1, Tatyana Makushok 1, Ulises Diaz 1, Wallace F. Marshall 1
1Department of Biochemistry and Biophysics, University of California San Francisco

The giant ciliate, Stentor coeruleus, is an excellent system to study regeneration and wound healing. We present procedures for establishing Stentor cell cultures from single cells or cell fragments, inducing regeneration by cutting cells, chemically inducing the regeneration of membranellar band and oral apparatus, imaging, and analysis of cell regeneration.

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Biology

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Lisa Couper 1, Andrea Swei 1
1Department of Biology, San Francisco State University

Here we present a next-generation sequencing protocol for 16S rRNA sequencing which enables identification and characterization of microbial communities within vectors. This method involves DNA extraction, amplification and barcoding of samples through PCR, sequencing on a flow-cell, and bioinformatics to match sequence data to phylogenetic information.

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Developmental Biology

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
Ulises Diaz 1,2, Wallace Marshall 2, Blake Riggs 1
1Department of Biology, San Francisco State University, 2Department of Biochemistry & Biophysics, UCSF Mission Bay

Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

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