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Ewha Womans University

6 ARTICLES PUBLISHED IN JoVE

Medicine

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Douglas Nam¹, Chih-Wen Ni², Amir Rezvan¹, Jin Suo², Klaudia Budzyn¹, Alexander Llanos¹, David G. Harrison¹, Don P. Giddens², Hanjoong Jo^1,2,3

¹Department of Medicine, Division of Cardiology, Emory University, ²Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, ³Department of Bioinspired Science, Ewha Womans University

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

Neuroscience

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Sang Beom Jun^1,2, Verginia Cuzon Carlson¹, Stephen Ikeda³, David Lovinger¹

¹Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, ²Department of Electronics Engineering, Ewha Womans University, ³Section on Transmitter Signaling/Laboratory of Molecular Physiology, National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism

This report demonstrates a technique for mechanical isolation of individual viable neurons retaining attached presynaptic boutons. Vibrodissociated neurons have the advantages of rapid production, excellent pharmacological control and improved space-clamp without influence from neighboring cells. This method can be used for imaging of synaptic elements and patch-clamp recording.

Behavior

Transcranial Direct Current Stimulation for Online Gamers

Sang Hoon Lee^*1, Jooyeon Jamie Im^*2, Jin Kyoung Oh², Eun Kyoung Choi², Sujung Yoon³, Marom Bikson⁴, In-Uk Song⁵, Hyeonseok Jeong², Yong-An Chung²

¹Department of Radiology, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ²Department of Radiology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Department of Brain and Cognitive Sciences, Ewha Womans University, ⁴Department of Biomedical Engineering, The City College of New York, ⁵Department of Neurology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea

We present a protocol and a feasibility study for applying transcranial direct current stimulation (tDCS) and neuroimaging assessment in online gamers.

JoVE Journal

Applying an eMASS Customization Program as a Research Tool to Evaluate Consumer Benefits

Minjung Park^*1, Jungmin Yoo^*2

¹Department of Fashion Industry, Ewha Womans University, ²Department of Business Administration, Duksung Women's University

Presented here is a protocol to examine consumer responses toward mass customization in the context of online retailing. The protocol details the online survey procedure and how to analyze data using structural equation modeling and group differences using latent mean analyses.

Biology

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Sandeep Kumar^*1, Aitor Andueza^*1, Nicolas Villa-Roel¹, Juyoung Kim¹, Dong-Won Kang¹, Hanjoong Jo^1,2

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²Division of Cardiology, Georgia Institute of Technology and Emory University

We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments.

Neuroscience

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

Youjin Lee^*1,2, Daeho Ryu^*3, Saeyeong Jeon³, Yena Lee¹, Yoon Kyung Cho¹, Chang-Hyeon Ji^1,2, Yong-Kweon Kim^3,4, Sang Beom Jun^1,2,5

¹Department of Electronic and Electrical Engineering, Ewha Womans University, ²Graduate Program in Smart Factory, Ewha Womans University, ³Department of Electrical and Computer Engineering, Seoul National University, ⁴Graduate School of Engineering Practice, Seoul National University, ⁵Department of Brain and Cognitive Sciences, Ewha Womans University

Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording.