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Rutgers Biomedical and Health Sciences

4 ARTICLES PUBLISHED IN JoVE

Medicine

An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

Lauren S. Sherman^1,2, Alexandra Condé-Green³, Yahaira Naaldijk¹, Edward S. Lee³, Pranela Rameshwar^1,2

¹Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School, Rutgers Biomedical and Health Sciences, ²Rutgers School of Graduate Studies at New Jersey Medical School, Rutgers Biomedical and Health Sciences, ³Department of Surgery, Division of Plastic Surgery, New Jersey Medical School, Rutgers Biomedical and Health Sciences

This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic.

Neuroscience

Stochastic Noise Application for the Assessment of Medial Vestibular Nucleus Neuron Sensitivity In Vitro

Sebastian P. Stefani^1,2, Paul P. Breen³, Jorge M. Serrador⁴, Aaron J. Camp^1,2

¹Discipline of Physiology, Sydney Medical School, University of Sydney, ²Bosch Institute, Sydney Medical School, University of Sydney, ³The MARCS Institute, Western Sydney University, ⁴Department of Pharmacology, Physiology & Neuroscience, Rutgers Biomedical and Health Sciences

Galvanic vestibular stimulation in humans exhibits improvements in vestibular function. However, it is unknown how these effects occur. Here, we describe how to apply sinusoidal and stochastic electrical noise and evaluate appropriate stimulus amplitudes in individual medial vestibular nucleus neurons in the C57BL/6 mouse.

Developmental Biology

Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

AnnJosette Ramirez^1,2, Sophie Astrof¹

¹Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers Biomedical and Health Sciences, ²Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School, Rutgers Biomedical and Health Sciences

Here, we describe a protocol to visualize and analyze the pharyngeal arch arteries 3, 4, and 6 of mouse embryos using whole-mount immunofluorescence, tissue clearing, confocal microscopy, and 3D reconstruction.

JoVE Core

Employing the Forced Oscillation Technique for the Assessment of Respiratory Mechanics in Adults

Wei Qian^1,2, Avani Desai³, Jennifer H. Therkorn¹, Jacquelyn C. Klein-Adams¹, Anays M. Sotolongo^1,2, Michael J. Falvo^1,2

¹Airborne Hazards and Burn Pits Center of Excellence, War Related Illness and Injury Study Center, VA NJ Health Care System, ²Rutgers New Jersey Medical School, Rutgers Biomedical and Health Sciences, ³Vassar College

As the use of forced oscillation technique (FOT) is increasingly utilized to characterize respiratory mechanics, there is a need to standardize methods with respect to nascent technical guidelines and various manufacturer's recommendations. A detailed protocol is provided including FOT assessment and interpretation for two cases to facilitate the standardization of methods.