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Saitama Medical Center, Jichi Medical University

5 ARTICLES PUBLISHED IN JoVE

Genetics

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Keiji Hirai¹, Hiromichi Yoshizawa², Toshimi Imai², Yusuke Igarashi², Ichiro Hirahara², Susumu Ookawara², Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Nephrology, Department of Internal Medicine, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

Here we present a protocol for the detection of microRNA expression in rat peritoneal membrane using quantitative real-time reverse-transcription polymerase chain reaction. This method is suitable for studying the microRNA expression profile in rat peritoneal membrane in several pathological conditions.

Genetics

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Katsunori Yanai¹, Shohei Kaneko¹, Hiroki Ishii¹, Akinori Aomatsu^1,2, Kiyonori Ito¹, Keiji Hirai¹, Susumu Ookawara¹, Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

We describe a method for evaluating the microRNA expression in the kidneys of mice with unilateral ureteral obstruction (UUO) by quantitative reverse-transcription polymerase chain reaction. This protocol is suitable for studying kidney microRNA expression profiles in mice with UUO and in the context of other pathological conditions.

Medicine

Measurement of Tissue Oxygenation Using Near-Infrared Spectroscopy in Patients Undergoing Hemodialysis

Kiyonori Ito^*1, Susumu Ookawara^*1, Takayuki Uchida², Hideyuki Hayasaka², Masaya Kofuji², Haruhisa Miyazawa¹, Akinori Aomatsu¹, Yuichiro Ueda¹, Keiji Hirai¹, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Department of clinical engineering, Saitama Medical Center, Jichi Medical University

We present a protocol to measure regional oxygen saturation (rSO₂) in hemodialysis (HD) patients by using a near-infrared spectroscopy monitor. The rSO₂ value is an index of tissue oxygenation. This noninvasive and real-time monitoring could be useful for confirming changes in organ oxygenation during HD.

Genetics

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Katsunori Yanai¹, Shohei Kaneko¹, Hiroki Ishii¹, Akinori Aomatsu^1,2, Kenichi Ishibashi³, Yoshiyuki Morishita¹

We present a method for evaluating microRNA expression in the kidney and serum of mice with age-dependent renal impairment by quantitative reverse-transcription polymerase chain reaction.

Medicine

Delivery of Exogenous Artificially Synthesized miRNA Mimic to the Kidney Using Polyethylenimine Nanoparticles in Several Kidney Disease Mouse Models

Katsunori Yanai^*1, Shohei Kaneko¹, Hiroki Ishii¹, Akinori Aomatsu¹, Yoshiyuki Morishita^*1

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University

Here, we deliver exogenous artificially synthesized miRNA mimics to the kidney via tail vein injection of a nonviral vector and polyethylenimine nanoparticles in several kidney disease mouse models. This led to significant overexpression of target miRNA in the kidney, resulting in inhibited progression of kidney disease in several mouse models.