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JoVE Journal

Bioengineering

This content is Open Access.

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

24.8K Views

10:33 min

February 23rd, 2018

February 23rd, 2018

24,762 Views

0:04

Introduction

0:56

Agarose Gel and Collagen Cell Model Preparation

2:42

Permeability Assay and Diffusion Simulation Setup

5:54

Parameter Optimization Addition

8:05

Results: Representative 3D Tissue Model Analyses

9:30

Conclusion

Transcrição

A method for determination of permeability in a membrane insert system for multi-well plates and in silico parameter optimization for the calculation of diffusion coefficients using simulation are presented.

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