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Chuncheon Bioindustry Foundation

3 ARTICLES PUBLISHED IN JoVE

Biochemistry

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass

Kenneth J. Olejar^*1, Min Hong^*2, Sun-Yeop Lee², Tae-Hyung Kwon², Soo-Ung Lee², Chad A. Kinney^1,3, Joon-Hee Han², Sang-Hyuck Park³

¹Department of Chemistry, Colorado State University-Pueblo, ²Chuncheon Bioindustry Foundation, ³Institute for Cannabis Research, Colorado State University-Pueblo

Ultrasonic-assisted extraction (UAE) increases extraction efficiency of solvents and when applied to Cannabis spp. biomass it reduces the time required for extraction. This decreases the cost and potential cannabinoid loss due to degradation. Additionally, UAE is considered a green method due to low solvent use.

Bioengineering

Employing Aeroponic Systems for the Clonal Propagation of Cannabis

Trevor Regas¹, Joon-Hee Han², Christopher S. Pauli³, Sang-Hyuck Park^1,3

¹Biology Department, Colorado State University-Pueblo, ²Chuncheon Bioindustry Foundation, ³Institute of Cannabis Research, Colorado State University-Pueblo

This protocol is designed to provide instructional information for the clonal propagation of Cannabis sativa L. by implementing aeroponic systems. The method described here includes all necessary supplies and protocols to successfully reproduce desirable morphological and chemical properties in the genus Cannabis.

Biology

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

Sang-Hyuck Park¹, Samuel Koch², Katherine Richardson³, Christopher Pauli¹, Joon-Hee Han⁴, Tae-Hyung Kwon⁴

¹Institute of Cannabis Research, Colorado State University-Pueblo, ²Department of Biology, Colorado State University-Pueblo, ³Department of Chemistry, Colorado State University-Pueblo, ⁴Chuncheon Bioindustry Foundation

The present protocol provides instructional information for using tobacco hornworm Manduca sexta in cannabinoid research. The method described here includes all necessary supplies and protocols to monitor physiological and behavioral changes of the insect model in response to cannabidiol (CBD) treatment.