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Chuncheon Bioindustry Foundation

3 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass
Kenneth J. Olejar *1, Min Hong *2, Sun-Yeop Lee 2, Tae-Hyung Kwon 2, Soo-Ung Lee 2, Chad A. Kinney 1,3, Joon-Hee Han 2, Sang-Hyuck Park 3
1Department of Chemistry, Colorado State University-Pueblo, 2Chuncheon Bioindustry Foundation, 3Institute for Cannabis Research, Colorado State University-Pueblo

Ultrasonic-assisted extraction (UAE) increases extraction efficiency of solvents and when applied to Cannabis spp. biomass it reduces the time required for extraction. This decreases the cost and potential cannabinoid loss due to degradation. Additionally, UAE is considered a green method due to low solvent use.

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Bioengineering

Employing Aeroponic Systems for the Clonal Propagation of Cannabis
Trevor Regas 1, Joon-Hee Han 2, Christopher S. Pauli 3, Sang-Hyuck Park 1,3
1Biology Department, Colorado State University-Pueblo, 2Chuncheon Bioindustry Foundation, 3Institute of Cannabis Research, Colorado State University-Pueblo

This protocol is designed to provide instructional information for the clonal propagation of Cannabis sativa L. by implementing aeroponic systems. The method described here includes all necessary supplies and protocols to successfully reproduce desirable morphological and chemical properties in the genus Cannabis.

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Biology

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies
Sang-Hyuck Park 1, Samuel Koch 2, Katherine Richardson 3, Christopher Pauli 1, Joon-Hee Han 4, Tae-Hyung Kwon 4
1Institute of Cannabis Research, Colorado State University-Pueblo, 2Department of Biology, Colorado State University-Pueblo, 3Department of Chemistry, Colorado State University-Pueblo, 4Chuncheon Bioindustry Foundation

The present protocol provides instructional information for using tobacco hornworm Manduca sexta in cannabinoid research. The method described here includes all necessary supplies and protocols to monitor physiological and behavioral changes of the insect model in response to cannabidiol (CBD) treatment.

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