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Howard Hughes Medical Institute, Johns Hopkins University School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

Hao Chang¹, Yanshu Wang¹, Hao Wu¹, Jeremy Nathans^1,2,3

¹Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, ²Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, ³Department of Ophthalmology, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine

Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.

Medicine

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment

Hao Wu¹, Ana R. Rodriguez², Bernd W. Spur², Venkat Venkataraman^1,2

¹Graduate School of Biomedical Sciences, Rowan University, ²Department of Cell Biology, Rowan University School of Osteopathic Medicine

A low-cost, easy-to-use and powerful system is established to evaluate potential treatments that could ameliorate blood retinal barrier breach induced by histamine. Blood vessel leakage, Müller cell activation and the continuity of neuronal processes are utilized to assess the damage response and its reversal with a potential drug, lipoxin A4.