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Mayo Clinic College of Medicine, Center for Individualized Medicine

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Choong Yong Ung¹, Feng Guo², Xiaoling Zhang³, Zhihui Zhu³, Shizhen Zhu^1,3

¹Department of Molecular Pharmacology & Experimental Therapeutics, Mayo Clinic College of Medicine, Center for Individualized Medicine, ²Tufts University School of Medicine, ³Department of Biochemistry and Molecular Biology, Mayo Clinic

The goal of this study is to demonstrate how the mosaic transgenesis strategy can be used in zebrafish to rapidly and efficiently assess the relative contributions of multiple oncogenes in tumor initiation and progression in vivo.

Cancer Research

Zebrafish Model of Neuroblastoma Metastasis

Zuag Paj Her¹, Kok Siong Yeo¹, Cassie Howe¹, Taylor Levee¹, Shizhen Zhu¹

¹Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Mayo Clinic Cancer Center

This paper introduces the method of developing, characterizing, and tracking in real-time the tumor metastasis in zebrafish model of neuroblastoma, specifically in the transgenic zebrafish line with overexpression of MYCN and LMO1, which develops metastasis spontaneously.