John Hunter Hospital
2 ARTICLES PUBLISHED IN JoVE
Biology
Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines
Brianna C. Morten 1,2, Rodney J. Scott 1,2,3, Kelly A. Avery-Kiejda 1,2
1Medical Genetics, Hunter Medical Research Institute, 2Priority Research Centre for Cancer, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, 3Pathology North, John Hunter Hospital
This protocol describes the use of three different methods for analyzing cell proliferation in breast cancer cell lines. This includes the use of conventional cell counting, luminescence-based cell viability, and cell counting through the use of a cell imager. Each offers advantages for the reproducible measurement of cell proliferation.
Cancer Research
A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
Xiajie Zhang 1,2, Brianna C. Morten 1,2, Rodney J. Scott 1,2,3, Kelly A. Avery-Kiejda 1,2
1Medical Genetics, Hunter Medical Research Institute, 2Priority Research Centre for Cancer Research, Innovation and Translation, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, 3Pathology North, John Hunter Hospital
The current protocol describes an integrated method investigating cancer cell migration and invasion on a single platform in real-time, providing an easily reproducible and time-efficient option to study cell mobility and morphology.
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