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John Hunter Hospital

2 ARTICLES PUBLISHED IN JoVE

Biology

Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines

Brianna C. Morten^1,2, Rodney J. Scott^1,2,3, Kelly A. Avery-Kiejda^1,2

¹Medical Genetics, Hunter Medical Research Institute, ²Priority Research Centre for Cancer, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, ³Pathology North, John Hunter Hospital

This protocol describes the use of three different methods for analyzing cell proliferation in breast cancer cell lines. This includes the use of conventional cell counting, luminescence-based cell viability, and cell counting through the use of a cell imager. Each offers advantages for the reproducible measurement of cell proliferation.

Cancer Research

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager

Xiajie Zhang^1,2, Brianna C. Morten^1,2, Rodney J. Scott^1,2,3, Kelly A. Avery-Kiejda^1,2

¹Medical Genetics, Hunter Medical Research Institute, ²Priority Research Centre for Cancer Research, Innovation and Translation, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, ³Pathology North, John Hunter Hospital

The current protocol describes an integrated method investigating cancer cell migration and invasion on a single platform in real-time, providing an easily reproducible and time-efficient option to study cell mobility and morphology.