Entrar

University of London

2 ARTICLES PUBLISHED IN JoVE

Research

Measuring Lactase Enzymatic Activity in the Teaching Lab

Cattleya S. Leksmono¹, Claudia Manzoni^1,2, James E. Tomkins¹, Walter Lucchesi^1,3, Graeme Cottrell¹, Patrick A. Lewis¹

¹School of Pharmacy, University of Reading, ²Department of Molecular Neuroscience, UCL Institute of Neurology, ³School of Biological Sciences, Royal Hollway, University of London

The enzymatic activity of lactase is essential for the catabolic processing of the disaccharide lactose. Here, the activity of lactase found in dietary supplements is assayed using a colorimetric assay. This provides students with an experimental platform for understanding the activity of lactase and enzyme kinetics.

Medicine

Cooling or Warming the Esophagus to Reduce Esophageal Injury During Left Atrial Ablation in the Treatment of Atrial Fibrillation

Jason Zagrodzky¹, Mark M. Gallagher², Lisa W. M. Leung², Tiffany Sharkoski³, Pasquale Santangeli⁴, Cory Tschabrunn⁴, Jose M. Guerra⁵, Bieito Campos⁵, John MacGregor⁶, Jamal Hayat², Brad Clark⁷, Alex Mazur⁸, Marcel Feher⁹, Martin Arnold⁹, Mark Metzl¹⁰, Jose Nazari¹⁰, Erik Kulstad¹¹

¹St. David's South Austin Medical Center, ²St George's University Hospitals NHS Foundation Trust, St. George's, University of London, ³Hospital of the University of Pennsylvania, Perelman Center for Advanced Medicine, ⁴University of Pennsylvania Perelman School of Medicine, ⁵Hospital de la Santa Creu I Sant Pau, Universitat Autònoma de Barcelona, CIBERCV, ⁶PeaceHealth Medical Group, St. Joseph Medical Center, ⁷St. Vincent Hospital, ⁸University of Iowa, ⁹University Hospital Erlangen, ¹⁰NorthShore University Health System, ¹¹Department of Emergency Medicine, University of Texas, Southwestern Medical Center

The goal of this protocol is to describe the use of esophageal temperature modulation to counteract esophageal thermal injury from left atrial ablation for the treatment of atrial fibrillation.