University of London
2 ARTICLES PUBLISHED IN JoVE
Research
Measuring Lactase Enzymatic Activity in the Teaching Lab
Cattleya S. Leksmono 1, Claudia Manzoni 1,2, James E. Tomkins 1, Walter Lucchesi 1,3, Graeme Cottrell 1, Patrick A. Lewis 1
1School of Pharmacy, University of Reading, 2Department of Molecular Neuroscience, UCL Institute of Neurology, 3School of Biological Sciences, Royal Hollway, University of London
The enzymatic activity of lactase is essential for the catabolic processing of the disaccharide lactose. Here, the activity of lactase found in dietary supplements is assayed using a colorimetric assay. This provides students with an experimental platform for understanding the activity of lactase and enzyme kinetics.
Medicine
Cooling or Warming the Esophagus to Reduce Esophageal Injury During Left Atrial Ablation in the Treatment of Atrial Fibrillation
Jason Zagrodzky 1, Mark M. Gallagher 2, Lisa W. M. Leung 2, Tiffany Sharkoski 3, Pasquale Santangeli 4, Cory Tschabrunn 4, Jose M. Guerra 5, Bieito Campos 5, John MacGregor 6, Jamal Hayat 2, Brad Clark 7, Alex Mazur 8, Marcel Feher 9, Martin Arnold 9, Mark Metzl 10, Jose Nazari 10, Erik Kulstad 11
1St. David's South Austin Medical Center, 2St George's University Hospitals NHS Foundation Trust, St. George's, University of London, 3Hospital of the University of Pennsylvania, Perelman Center for Advanced Medicine, 4University of Pennsylvania Perelman School of Medicine, 5Hospital de la Santa Creu I Sant Pau, Universitat Autònoma de Barcelona, CIBERCV, 6PeaceHealth Medical Group, St. Joseph Medical Center, 7St. Vincent Hospital, 8University of Iowa, 9University Hospital Erlangen, 10NorthShore University Health System, 11Department of Emergency Medicine, University of Texas, Southwestern Medical Center
The goal of this protocol is to describe the use of esophageal temperature modulation to counteract esophageal thermal injury from left atrial ablation for the treatment of atrial fibrillation.
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