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UMC Utrecht

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

Vahid Arbabi^*1,2, Behdad Pouran^*1,2, Amir. A. Zadpoor¹, Harrie Weinans^1,2,3

¹Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology (TU Delft), ²Department of Orthopedics, UMC Utrecht, ³Department of Rheumatology, UMC Utrecht

We propose a protocol to investigate the transport of charged and uncharged molecules across articular cartilage with the aid of recently developed experimental and numerical methods.

Immunology and Infection

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

Devanjali Dutta¹, Inha Heo^1,3, Roberta O'Connor²

¹Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), UMC Utrecht, ²Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, ³Janssen Pharmaceutica

We describe protocols to prepare oocysts and purify sporozoites for studying infection of human intestinal and airway organoids by Cryptosporidium parvum. We demonstrate the procedures for microinjection of parasites into the intestinal organoid lumen and immunostaining of organoids. Finally, we describe the isolation of generated oocysts from the organoids.

Neuroscience

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Cristina Melero^*1, Aljona Kolmogorova^*1, Paul Atherton¹, Brian Derby², Adam Reid^3,4, Karin Jansen⁵, Christoph Ballestrem¹

¹Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research, The University of Manchester, ²School of Materials, The University of Manchester, ³Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester, Manchester Academic Health Science Centre, ⁴Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, ⁵Department of Pathology, UMC Utrecht

Our overall aim is to understand how cells sense extracellular cues that lead to directed axonal growth. Here, we describe the methodology of Light-Induced Molecular Adsorption of Proteins, used to produce defined micro-patterns of extracellular matrix components in order to study specific events that govern axon outgrowth and pathfinding.