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5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

¹Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston

We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.

JoVE Core

Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes

Du Zhou¹, Xi Yuan¹, Haoxiang Gao¹, Ailing Wang¹, Jun Liu¹, Omer El Fakir¹, Denis J. Politis¹, Liliang Wang¹, Jianguo Lin¹

¹Department of Mechanical Engineering, Imperial College London

The following paper presents a novel FE simulation technique (KBC-FE), which reduces computational cost by performing simulations on a cloud computing environment, through the application of individual modules. Moreover, it establishes a seamless collaborative network between world leading scientists, enabling the integration of cutting edge knowledge modules into FE simulations.

Developmental Biology

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Shen Zhang¹, Yonggen Wu¹, Yimin Weng², Zhihui Xu¹, Wenmin Chen³, Dahan Zheng⁴, Wei Lin⁵, Jun Liu⁶, Ying Zhou^1,7

¹Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, ²Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, ³Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, ⁴School of Laboratory Medicine and Life Science, Wenzhou Medical University, ⁵School of Pharmaceutical Science, Wenzhou Medical University, ⁶Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, ⁷Department of Histology and Embryology, Wenzhou Medical University

A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.

Bioengineering

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement

Jun Liu¹, John Wilson², Claire Davis¹, Anthony Peyton²

¹Advanced Steel Research Centre, Warwick Manufacturing Group, University of Warwick, ²School of Electrical and Electronic Engineering, University of Manchester

This paper elaborates the sample and sensor preparation procedures and the protocols for using the test rig particularly for dynamic domain imaging with in situ BH measurements in order to achieve optimal domain pattern quality and accurate BH measurements.

Immunology and Infection

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

David Chetrit¹, Donghyun Park^1,2, Bo Hu³, Jun Liu^1,2, Craig R. Roy¹

¹Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, ²Microbial Sciences Institute, Yale University, ³Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston

Imaging of bacterial cells is an emerging systems biology approach focused on defining static and dynamic processes that dictate the function of large macromolecular machines. Here, integration of quantitative live cell imaging and cryo-electron tomography is used to study Legionella pneumophila type IV secretion system architecture and functions.