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Chinese Academy of Medical Science & Peking Union Medical College

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating
Jingyi Yang 1, Shaofeng Lou 1, Deling Kong 1, Chen Li 1
1Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College

This protocol aims to achieve surface engineering of pancreatic islets using a heparin-incorporated starPEG nanocoating via pseudo-bioorthogonal chemistry between the N-hydroxysuccinimide groups of the nanocoating and the amine groups of islet cell membrane.

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Biology

Implantation of Electrospun Vascular Grafts with Optimized Structure in a Rat Model
Kang Qin 1, Yifan Wu 1, Yiwa Pan 1, Kai Wang 1, Deling Kong 1, Qiang Zhao 1
1State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials (Ministry of Education), College of Life Sciences, Nankai University

Here, we present a modified electrospinning method to fabricate PCL vascular grafts with thick fibers and large pores, and describe a protocol to evaluate the in vivo performance in a rat model of abdominal aorta replacement.

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Medicine

Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots
Ruihao Li 1,2, Zuoguan Chen 1, Yaming Guo 1,2, Changduo Wang 3, Yongpeng Diao 1, Jing Yang 3, Yongjun Li 1,2
1Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, 2Graduate School of Chinese Academy of Medical Sciences & Peking Union Medical College, 3Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College

A detailed methodology for establishing a minimally invasive rat model of pulmonary embolism using autologous blood clots is described. Additional methods for quantifying the infarcted area and visualizing the pulmonary arterial tree are also provided.

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