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Shanghai Jiao Tong University, School of Medicine

8 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Sara Dolatshahi Pirooz¹, Joo-Hyung Lee¹, Zhen Zhao¹, Duojiao Ni¹, Soohwan Oh¹, Chengyu Liang¹

¹Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles

γ-Herpesviruses (γ-HVs) establish life-long persistency in their host. Infection of mice with γ-HV68 provides a genetically tractable in vivo model for the characterization of the lifecycle/pathogenesis of γHVs. This protocol describes the detection and quantitation of γHV68 infection at acute and latent stages following infection by plaque-forming, infectious center, and qPCR assays.

Developmental Biology

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Ieng Lam Lei¹, Lei Bu², Zhong Wang¹

¹Cardiac Surgery, University of Michigan, ²Leon H Charney Division of Cardiology, New York University School of Medicine

In this protocol, derivation of cardiac progenitor cells from both mouse and human embryonic stem cells will be illustrated. A major strategy in this protocol is to enrich cardiac progenitor cells with flow cytometry using fluorescent reporters engineered into the embryonic stem cell lines.

Medicine

Surgical Treatment for Benign Prostatic Hyperplasia: Holmium Laser Enucleation of the Prostate (HoLEP).

Huan Xu^*1, Xiang Wan^*1, Meng Gu¹, Yanbo Chen¹, Qiling Shi¹, Qi Chen¹, Zhong Wang¹

¹Department of Urology, Shanghai 9th People's Hospital, Shanghai Jiaotong University School of Medicine

Here we present a safe and efficient protocol, holmium laser enucleation of the prostate, to treat benign prostatic hyperplasia.

Immunology and Infection

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Tanja Vajen¹, Alexandra C.A. Heinzmann¹, Annemiek Dickhout¹, Zhen Zhao², Magdolna Nagy¹, Johan W.M. Heemskerk¹, Rory R. Koenen¹

¹Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, ²Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine

Leukocytes avidly interact with vascular cells and platelets after vessel wall injury or during inflammation. Here, we describe a straightforward laminar flow-based assay to characterize the molecular mechanisms that underlie the interactions between leukocytes and their cellular partners.

Cancer Research

A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity

Yanlan Wang^1,2, Jiayu Liu^1,2, Haitao Pan^1,2, Jieyu Xing^1,2, Xiaoqiong Wu^1,2, Qing Li^1,2, Zhong Wang^1,2

¹School of Pharmaceutical Sciences, Sun Yat-Sen University, ²Center for Cellular & Structural Biology, Sun Yat-Sen University

Here, we present a protocol to produce a bispecific antibody GPC3-S-Fab in Escherichia coli. The purified GPC3-S-Fab has potent cytotoxicity against GPC3 positive liver cancer cells.

Chemistry

A New Straightforward Method for Lipophilicity (logP) Measurement using ¹⁹F NMR Spectroscopy

Zhong Wang¹, Benjamin F. Jeffries¹, Hannah R. Felstead¹, Neil J. Wells¹, Elisabetta Chiarparin², Bruno Linclau¹

¹Chemistry, University of Southampton, ²Medicinal Chemistry, Oncology, IMED Biotech Unit, AstraZeneca

A novel and straightforward variation of the shake-flask method was developed for accurate lipophilicity measurement of fluorinated compounds by ¹⁹F NMR spectroscopy.

Medicine

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Zhaokai Li^*1,2, Liu Liu^*1, Zhong Wang¹

¹Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, ²Department of Cardiovascular Medicine, Xiangya Hospital, Central South University

We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sor^{tm38(CAG-GCaMP3)Hze}/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca²⁺) flux.

Medicine

A Modified Bilateral Scrotal Flap for Penile Skin Defect Repair

Haijun Yao^*1, Dachao Zheng^*1, Minkai Xie¹, Yuanshen Mao¹, Xiang Wan¹, Jianshu Ni¹, Zhong Wang¹

¹Department of Urology, Shanghai 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine

This study presents an application of the modified bilateral scrotal flap for the reconstruction of penile skin shortages and scar contractures.